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This Siedentopf trinocular microscope is ideal for use at the senior high school and college level, or for use in veterinary and medical applications. The adjustable interpupillary distance ranges from 54mm to 76mm, and it offers four levels of magnification, ranging from 40x to 1000x. The trinocular port allows you to connect a digital camera or an LCD screen, to broadcast images of the specimen being observed. Eyepiece with a built-in pointer ensures that definite points on the slide can be easily designated and quickly identified. Standard DIN achromatic objectives are parfocaled, parcentered, and color-coded to provide sharp, color-corrected images. The coaxial coarse and fine focusing knobs are positioned low on the bracket, near the base, making certain that adjustments are made with maximum stability and minimum strain. The ball-bearing driven, reversed nosepiece with internal click-stops, guarantee parcentration and repeatability with every magnification change.
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Compound Microscopes
Specifications Packing List Suggested Accessories Quick Overview Infinite. Total Magnification: 40-1000X. 10X Eyepiece. 4X 10X 40X 100X Infinity Plan Achromatic Objective. Standard Coupler: 1X. Eye Tube Angle: 30°. XY Stage Travel Distance: 75x50mm. Stage Scale: X: 0-68mm Y: 0-65mm. Illumination Type: LED Coaxial Transmitted Light. Camera Adapter Magnification: 1X. Input Voltage: AC 110-240V 50/60Hz. Suggested Applications Education & Student , Multi-Head and Training BM13010533 5-Head Viewing Teaching Compound Microscope Optical System Specifications Optical System Infinite Tube Lens Focal Length 180mm System Optical Magnification 40-1000X Expandable System Optical Magnification (Optional Parts Required) 40-1600X Trinocular Optical Magnification 4-100X Total Magnification 40-1000X Standard Eyepiece 10X Eyepiece Standard Objective 4X 10X 40X 100X Infinity Plan Achromatic Objective Standard Coupler 1X System Field of View Dia. 0.25-5mm Expandable System Field of View Dia. 0.12-5.5mm System Working Distance 0.21-25.42mm Expandable System Working Distance 0.16-25.42mm Compound Trinocular Head Eye Tube Optical System Infinite Eye Tube Type For Compound Microscope Eye Tube Adjustment Mode Siedentopf Eye Tube Angle 30° Erect/Inverted Image Inverted Image Eye Tube Rotatable 360° Degree Rotatable Interpupillary Adjustment 48-75mm Eye Tube Inner Diameter Dia. 30mm Eye Tube Diopter Adjustable Left ±5°, Right Not Adjustable Eye Tube Size for Scope Body/Carrier Dia. 42.5x6mm Image Port Switch Mode 0/100 Switch Trinocular Surface Treatment Spray Paint Material Metal Color White Net Weight 1.24kg (2.73lbs) Applied Field For BM1301 Series Microscope Biological Objective 4X Infinity Plan Achromatic Objective Objective Optical System Infinite Objective Optical Magnification 4X Objective Type Plan Achromatic Objective Objective Parfocal Distance 45mm Objective for Focal Length 180mm Objective Working Distance 25.42mm Numerical Aperture (N.A.) N.A. 0.10 Objective Cover Glass Thickness /0 Objective Immersion Media Dry Objective Objective Screw Thread RMS Standard (4/5 in. x1/36 in. ) Objective Outer Diameter Dia. 24.5mm Surface Treatment Polished Chrome Material Metal Color Silver Net Weight 0.07kg (0.15lbs) Applied Field BM1301, FM1301 Series Microscope 10X Infinity Plan Achromatic Objective Objective Optical System Infinite Objective Optical Magnification 10X Objective Type Plan Achromatic Objective Objective Parfocal Distance 45mm Objective for Focal Length 180mm Objective Working Distance 11mm Numerical Aperture (N.A.) N.A. 0.25 Objective Cover Glass Thickness 0.17 Objective Immersion Media Dry Objective Objective Screw Thread RMS Standard (4/5 in. x1/36 in. ) Objective Outer Diameter Dia. 24.5mm Surface Treatment Polished Chrome Material Metal Color Silver Net Weight 0.08kg (0.18lbs) Applied Field BM1301, FM1301 Series Microscope 40X Infinity Plan Achromatic Objective Objective Optical System Infinite Objective Optical Magnification 40X Objective Type Plan Achromatic Objective Objective Parfocal Distance 45mm Objective for Focal Length 180mm Objective Working Distance 0.75mm Numerical Aperture (N.A.) N.A. 0.65 Objective Cover Glass Thickness 0.17 Objective Immersion Media Dry Objective Spring Mounted Objective Spring Mounted objective Objective Screw Thread RMS Standard (4/5 in. x1/36 in. ) Objective Outer Diameter Dia. 24.5mm Surface Treatment Polished Chrome Material Metal Color Silver Net Weight 0.12kg (0.26lbs) Applied Field BM1301, FM1301 Series Microscope 100X Infinity Plan Achromatic Objective Objective Optical System Infinite Objective Optical Magnification 100X Objective Type Plan Achromatic Objective Objective Parfocal Distance 45mm Objective for Focal Length 180mm Objective Working Distance 0.21mm Numerical Aperture (N.A.) N.A. 1.25 Objective Cover Glass Thickness 0.17 Objective Immersion Media Oil Immersion Objective Spring Mounted Objective Spring Mounted objective Objective Screw Thread RMS Standard (4/5 in. x1/36 in. ) Objective Outer Diameter Dia. 24.5mm Surface Treatment Polished Chrome Material Metal Color Silver Net Weight 0.13kg (0.29lbs) Applied Field BM1301, FM1301 Series Microscope Nosepiece Inward/Outward Nosepiece Nosepiece Inward Number of Holes on Nosepiece Quintuple (5) Holes Nosepiece Switch Mode Manual Nosepiece Screw Thread for Objective RMS Standard (4/5 in. x1/36 in. ) Microscope Stand Stand Height 300mm Base Type Illumination Base Base Shape Triangle Base Dimensions 320x270x80mm/ Dia. 140mm Focus Mode Manual Coarse/Fine Focus Type Coaxial Coarse/Fine Focus Focus Distance 17.5mm Fine Focus Travel Distance Same as Focus Distance Coarse Focus Distance per Rotation 19.4mm Fine Focus Distance per Rotation 0.1mm Fine Focus Minimum Scale 1μm Focus Limited Limited Focusing Knob Tightness Adjustable Tightness Adjustable Microscope Stage XY Stage Travel Distance 75x50mm XY-Axis Drive Mode Manual Stage Platform Dimensions 185x140mm Stage Backlight Window Size 30x81mm Stage Scale X: 0-68mm Y: 0-65mm Opening Size of Stage Specimen Holder Opening 55-95mm Microscope Illuminator Illumination Type LED Coaxial Transmitted Light Transmission Light Kohler Illumination Transmission Light Source Type LED Aperture Diaphragm Mounting Position Vertical Illuminator Aperture Diaphragm Outer Diameter Dia. 61mm Field Diaphragm Mounting Position Vertical Illuminator Field Diaphragm Outer Diameter Dia. 86mm Number of Filter Slots 1 Filter Switch Type Embedded Type Condenser Condenser Type Swing Out Condenser Dry/Oil Type Dry Applicable Range of Objective 4-100X Condenser Adjustable Adjustable Condenser Top Lens Swing-out Top Lens Condenser Max. Numerical Aperture N.A. 0.90 Condenser Mounting Flange Size Fastening Screw Surface Treatment Electroplating Black Material Metal Color Black Net Weight 0.11kg (0.24lbs) Applied Field BM1301, FM1301 Series Microscope C
olor Filter Filter (Blue) Filter Color Blue Filter Size Dia. 45mm Filter Switch Type Embedded Type Material Glass Net Weight 0.008kg (0.018lbs) Applied Field For BM1301, BM1302 Series Microscope Filter (Green) Filter Color Green Filter Size Dia. 45mm Filter Switch Type Embedded Type Material Glass Net Weight 0.008kg (0.018lbs) Applied Field For BM1301, BM1302 Series Microscope Coupler/C-mount Adapter 1X Coupler Coupler Mount Type for Trinocular Fastening Screw Coupler Mount Size for Trinocular Dia. 38mm Coupler for Microscope Type Compound Compatible Coupler Magnification 1X For Camera Sensor Size Under 2/3 in. C/CS-Mount Coupler C-Mount Surface Treatment Electroplating Black Material Metal Color Black Applied Field For FM1301, PL1301, MT1301, MT1302, MT1303, MT1304, BM1301, BM1302, BM1303, BM1306 Series Microscope Digital Camera Adapter Coupler Adapter Camera Adapter Magnification 1X Adapter Mount Size for DSLR Camera Dia. 23.2mm Adapter Mount Size for Microscope Dia. 24mm Surface Treatment Electroplating Black Material Metal Color Black Net Weight 0.13kg (0.29lbs) Applied Field For FM1301, PL1301, MT1301, MT1302, MT1303, MT1304, BM1301, BM1302, BM1303, BM1306 Series Microscope Camera Conversion Adapter Coupler Adapter Conversion Adapter Size Dia. 38/38mm Surface Treatment Spray Paint Material Aluminum Color White Net Weight 0.19kg (0.42lbs) Applied Field For FM1301, PL1301, MT1301, MT1302, MT1303, MT1304, BM1301, BM1303, BM1306 Series Microscope Power Supply Input Voltage AC 110-240V 50/60Hz Power Cord Connector Type USA 3 Pins Power Cable Length 1.8m Laser Pointer Pointer Type Built-in System Pointer Light Waves Type Laser Pointer Light Color Green Pointer Light Shape Arrow Other Parameters Surface Treatment Spray Paint Material Metal Color White Net Weight 22.53kg (49.107lbs) Dimensions 1350x400x560mm (53.150×15.748×22.047 in. ) Series BM1301 BM13010533 Technical Info Instructions Biological MicroscopeOpen V Biological microscopes are compound microscopes that are primarily used to observe and study organisms and microorganisms. Biological microscopes were the earliest type of microscopes to be invented and the most widely used compound microscope today. Humans first used simple microscopes to observe tiny objects with a lens. Later, compound microscope were invented, which then used two lenses, i.e., one eyepiece and one objective lens for secondary imaging, to obtain a larger multiple of the image. Conventionally, we usually refer to microscopes that include various accessories such as phase contrast, fluorescence, and polarized light etc. as compound microscopes, to distinguish them from stereo microscopes. (Although stereo microscopes also have an eyepiece and an objective lens, they have two light paths, which presents a three-dimensional image). The most basic biological microscope consists of an eyepiece, an objective lens, a microscope stage, and light source. Both the eyepiece and the objective lens are convex lenses. The objective lens first enlarges the object into a real image. The eyepiece then magnifies the real image again into a virtual image, and finally becomes an inverted magnified virtual image on the retina of the human eye. Biological microscopes are usually used to observe transparent or translucent objects, such as animal and plant cells, tissues, bacteria and microorganisms, as well as various kinds of tiny particles by means of sectioning. They are widely used in teaching, medicine, animal or plant research and industrial fields. Modern optical microscopes have made great progress in the wavelengths of various kinds of light; illumination forms, resolution, microscope functions, structure and comfort of image acquisition and analysis, and basically meet various research needs. According to the user’s needs and the complexity of the product, general biological microscopes are divided into student-level, experimental-grade, and research-level biological microscopes. Basic Structure of Biological Microscope A standard biological microscope usually has at least the following basic structures: 1. Objective lens – the closest imaging lens to the observed specimen. Objective lens determines the most important properties of the microscope imaging; such as wavelength and resolution of the object light. A microscope can have several objective lenses with different magnifications. 2. Eyepiece – the lens mounted on the upper end of the microscope tube; close to the observer’s eyes. Generally, microscopes can have several eyepieces with different magnifications. 3. Light source – the light source of the biological microscope is under the microscope stage. According to different needs, a light source may include an illuminating light source (bulb), an aperture diaphragm, a condenser etc. The condenser is used to condense the illumination light and also increase the illumination brightness of the specimen. Aperture diaphragm, also called iris, is used to adjust the luminous flux of light. Under the aperture diaphragm, there is usually a circular filter holder, and the optical filters are placed according to needs. A simple microscope would not have an illuminating light source, it is illuminated by natural light, and a reflector is used to illuminate the object to be observed. 4. Microscope base – located at the bottom of the microscope; to support the lens body. Usually, the light source and the electrical appliances are installed inside the base and above the base. 5. Microscope body – used to connect and stand the various components of the entire microscope, and it is also the part the user holds when moving the microscope. 6. Microscope tube – an optical path channel connecting the eyepiece and the nosepiece of the microscope. 7. Nosepiece – the turntable under the microscope tube. The nosepiece usually has 3 to 4 circular holes for mounting objective lenses of different magnification; which can be rotated onto the optical axis of the microscope for use. 8. Microscope stage – where the specimen is placed for observation. There are usually two metal tablets on the mobile station, which are used to fix the specimen of the slide. There is also usually a pusher installed for moving the specimen. There are also microscope stages that can be moved directly in the XY direction. 9. Focus knob – used to adjust the distance between the objective lens and the microscope stage (sample) to bring the objective lens into focus to get a clear picture or image. The focus knob is usually mounted with the microscope stage to achieve the purpose of moving up and down focusing through the coarse focus knob and the fine focus knob. Biological Microscope Quick Operation Steps Step 1. Install and Prepare: The configuration of the biological microscope is mostly standard. Carefully check the parts on the packing list and the information on the BoliOptics website to assemble and install the microscope. The microscope should be placed on a solid and stable work surface with the tabletop kept steady, clean, and close to a power source. It is best to place the microscope out of direct sunlight. Generally speaking, the darker the environment, the better the image is observed by the microscope. Stray light greatly influences the imaging when the microscope is used for observation, as it can damage the specimen and can also accelerate the aging of the microscope surface and components. Step 2. Turn on the light source: Connect the power source, turn on the power switch, and adjust the light source to a position where the brightness is moderate. Step 3. Place the specimen (also known as the type or sample): Adjust the coarse focus knob first, and raise the objective lens to a higher position for easy placement of the specimen. Place the slide specimen of the observed object on the microscope stage. Note that the side of the cover slip is placed face up. Then use spring pressure to clamp on both ends of the slide to prevent the specimen from moving, and then adjust the knob through the XY direction of the microscope stage to move the general position of the part of the specimen to be observed to the center of the condenser. Step 4. Adjust the parf
ocal of the high and low objective lens: First observe with low power objectives. Adjust the low power lens (such as 4X, 10X) from the objective lens or nosepiece to the optical axis. Then, adjust the focus knob to find the outline of the image. Because the low power objectives have a large field of view, it is easier to find the image and determine the part to be observed. At the same time, adjust the XY microscope stage hand button to find the position of the specimen to be observed. It should be noted that the image of the biological microscope in the field of view is usually an inverted image, that is, the specimen should be moved in the opposite direction when moving the specimen. Then, turn the nosepiece and gradually use the high power objective (such as 40X) to move to the observation position, and finally to the maximum magnification (such as 100X). During the process, continually adjust the fine adjustment knob to find the clearest image. With regard to the observation and use of the oil lens, it is generally carried out after the above steps, and finally make further accurate observation. When changing from low power objectives to high power objectives, the object image can generally be seen, but it may not be very clear. When rotating to the maximum power objectives (such as 100X), only the fine focus knob should be used rather than the coarse focus knob, so as to avoid damage to the lens or the slide specimen. When the image of the maximum power objective is clear using a microscope with normal function, ensure that the low power objectives and the high power objectives are parfocal, and the focus knob is no longer adjusted. During operation, it is possible that the power of some of the objectives in the middle may not be parfocal. If so, you only need to adjust the fine focus knob slightly. Using a binocular microscope – If the observer’s binocular vision is different, adjust it by the eyetube diopter of the eyepieces. Do not adjust the focus knob. Step 5. Adjust the Light Source: Adjust the light intensity of the light source. Adjust the size of the diaphragm, the height of the condenser, the angle of the reflector. These adjustments need to be coordinated and adjusted with the power of objective in order to get a clear image. Under normal circumstances, the light of the stained specimen should be strong, and the light of the colorless or unstained specimen should be dim. When adjusting between high and low power objectives, the light for low power objectives for observation should be dim, and the light for high power objectives for observation should be strong. Step 6. Replace the specimen: After observing the specimen – if you need to switch to another slide, you should first change the objectives back to low power, remove the slide before replacing it with a new one, and then adjust the focus again for observation. Do not change the specimen under the high power objectives as the working distance is very small, so as to prevent damage to the objective lens. Step 7. Arranging the microscope after use: After observing with the microscope, the objective lenses should be moved away from the light-passing hole. Turn the nosepiece so that the V-shape between the lenses is slanted to both sides. Remove the sample. Check the light source of the microscope – adjust the aperture diaphragm to the maximum, adjust the brightness knob to the darkest, and then turn off the power button to prevent the instantaneous high current from burning out the light source when the power is turned on next time. Lower the microscope stage and check if any parts are damaged, if the objective lens is stained with water or oil, or if the objective body has stains or hand prints. Wipe the microscope clean, and check that the accessories are complete, the sample specimens are complete, and anything else is complete. After the final inspection is completed, cover the microscope with a dust cover or place the microscope into a box. Biological microscopes are the basic structure of other forms of compound microscopes that are added with various kinds of accessories or attachments. Many principles and key points are fundamentally reflected in biological microscopes. InfiniteClose Λ Microscopes and components have two types of optical path design structures. One type is finite optical structural design, in which light passing through the objective lens is directed at the intermediate image plane (located in the front focal plane of the eyepiece) and converges at that point. The finite structure is an integrated design, with a compact structure, and it is a kind of economical microscope. Another type is infinite optical structural design, in which the light between the tube lens after passing the objective lens becomes \
